International Journal of Clinical Embryology and Reproductive Biology
International Journal of Clinical Embryology and Reproductive Biology is a peer-reviewed, quarterly journal dedicated to publishing high-quality research, reviews, and advancements in the field of clinical embryology and reproductive biology. The journal provides a platform for researchers, clinicians, and specialists to share their latest findings, innovations, and insights into human reproduction, assisted reproductive technologies (ART), infertility treatments, and related biomedical sciences. Published in English, the journal welcomes contributions that enhance scientific understanding and clinical applications in reproductive medicine, embryology, and fertility studies. It aims to bridge the gap between research and clinical practice, supporting the development of new techniques and improving reproductive health outcomes worldwide.
Department of Biochemistry And Forensic Science, Gujarat University
TOXICO-METABOLOMIC SIGNATURES OF BLOOD PLASMA AND SEMINAL PLASMA IN INFERTILE MALES USING LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
1.1. Background and Challenge Infertility in males represents a worldwide substantial public health challenge, affecting millions of couples globally. Idiopathic and unexplained infertility remains the most frustrating subset of the lot as assigning a clear etiology becomes a challenge by routine diagnostic procedures.22 this decline in semen quality is justified, partially, by global scientific evidences suggesting the detrimental role of acute and chronic exposures of environmental as well as occupational xenobiotics such as Organophosphates (OP) and endocrine disrupting chemicals (EDCs) in reproductive health.3 which leads to a critical need for identification of non-invasive but quantifiable biomarkers that can bridge the gap between toxic exposures and their impairing effects on functional reproductive health. 1.2. Methodology This study follows on the toxico-metabolomic profiling conducted on matched blood plasma (BP) and seminal plasma (SP) samples collected from infertile patients exhibiting abnormal semen parameters (Oligo- Astheno- Teratozoospermia, OAT). Samples were prepared using high-throughput Toxtyper® liquid- liquid extraction (LLE) protocol and analysed using high resolution liquid chromatography- tandem mass spectrometry (LC-MS/MS) on a Bruker amazon speed ion trap instrument. The rapid and simultaneous detection of broad spectrum of small molecule’s, including basic, acidic and neutral compounds was ensured and encouraged by the fast polarity switching capabilities of the method. 1.3. Key Results The toxico-metabolomic analysis successfully identified a co-occurring molecular signature consisting of nine distinct xenobiotics across the study cohort. High-priority compounds detected included the potent EDCs Di(2-ethylhexyl) phthalate (DEHP) and Tetrabromobisphenol A (TBBPA), along with the OP insecticide Acephate. Comparative quantification revealed that eight of the nine identified toxins were present in both the systemic (BP) and reproductive (SP) compartments. Analysis of molecular abundance demonstrated a significant transfer rate of these lipophilic compounds into the seminal microenvironment, often reaching concentrations hypothesized to interfere with germ cell function. 1.4. Conclusion The toxico-metabolomic profile provides a novel and quantifiable etiology of previous idiopathic male infertility by confirming the direct exposure of germ sells to reproductive toxicants via seminal fluid. This finding validates the use of seminal plasma analysis as a critical, non-invasive diagnostic tool by offering molecular and targeted basis of clinical intervention for similar cases of environmental or occupational toxicity on reproductive functions.21
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EFFECT OF INTRAOVARIAN PRP ON THE QUALITY AND QUANTITY OF OOCYTES, AND FERTILIZATION RATE
Infertility is on a rise in the current scenario. There are various factors that contribute to infertility. One of them is ovarian dysfunction. Ovarian dysfunction includes PCOS, ovarian aging, etc. Ovarian aging is a physiological process wherein the antral follicle count decreases thereby leading to decrease in the quantity and quality of the oocytes and causing menopause in females. Premature ovarian ageing is increasing in females who are below the menopausal age. In order to restore the ovarian reserve, one of the therapeutic treatments that is being used is autologous platelet rich plasma therapy. The platelets are rich in growth factors, integrins, chemokines and cytokines which promote cell division and differentiation, angiogenesis, cell migration, tissue repair and restoration. The platelets are extracted from the whole blood and are then injected into ovaries. Following the injection, the stimulation is started from the next cycle and the IVF procedure is performed. The objective of our study is to see the effect of PRP on the quality and quantity of oocytes, fertilization rate and implantation rate and to compare it with IVF cycle done prior to PRP. It was observed that there was an increase in the quality and quantity of oocytes and fertilization rate in post PRP IVF cycles compared to Pre cycles. The significance level of our study is above 95% hence proving the data statistically significant.
Infertility, IntraOvarian, Autologous Platelet Rich Plasma Therapy, Premature Ovarian failure.
Infertility is on a rise in the current scenario. There are various factors that contribute to infertility. One of them is ovarian dysfunction. Ovarian dysfunction includes PCOS, ovarian aging, etc. Ovarianagingisaphysiologicalprocesswhereintheantralfolliclecountdecreases thereby leading to decrease in the quantity and quality of the oocytes and causing menopause in females. Premature ovarian ageing is increasing in females who are below the menopausal age. Inordertorestoretheovarianreserve,oneofthetherapeutictreatmentsthatisbeingused is autologous platelet rich plasma therapy. The platelets are rich in growth factors, integrins, chemokines and cytokines which promote cell division and differentiation, angiogenesis, cell migration, tissue repair and restoration. The platelets are extracted from the whole blood and are then injected into ovaries. Following the injection, the stimulation is started from the next cycle and the IVF procedure is performed. The objective of our study is to see the effect of PRP on the quality and quantity of oocytes, fertilization rate and implantation rate and to compareitwithIVFcycledonepriortoPRP.Itwasobservedthattherewasanincreaseinthe quality and quantity of oocytes and fertilization rate in post PRP IVF cycles compared to Pre cycles. The significance level of our study is above 95% hence proving the data statistically significant.
Infertility,IntraOvarian,AutologousPlateletRichPlasmaTherapy,Premature Ovarian failure
EVALUATING EFFECTS OF LOW SERUM AMH ON OOCYTE MATURATION, EMBRYO DEVELOPMENT AND CLINICAL OUTCOME IN ASSISTED REPRODUCTIVE TECHNIQUES
In the current scenarios, female infertility is on a rise. There are many causes of female infertility, however, Low Serum AMH leading to Poor Ovarian Reserve is one of the leading cause of infertility in females with age 35 or less. AMH also known as Anti Müllerian Hormone is one of the key hormone for ovarian reserve markers. A lower level of AMH, often indicates poor ovarian reserve. A correlation between the low level of serum AMH, oocyte quality and quantity, embryo quality and clinical outcome is unclear yet. This study aims in finding out the correlation between the low level of serum AMH, oocyte quality and quantity, embryo quality and clinical outcome. A short retrospective cohort study is done and the patients are categorized in two groups – Group I having patients with low AMH (less than 1.1 ng/ml) and Group II having patients with normal AMH (more than 1.1 ng/ml). The oocyte quality and quantity, embryo quality and clinical outcome of the patients are assessed and statistical analysis is performed. The results indicate that there is a correlation observed between low serum AHM levels, oocyte quality and quantity, embryo quality and clinical outcome. With lower levels of AMH, there is a decrease in the quantity of oocytes as well as there is a decrease in the quality of oocyte and embryo, leading to an overall decrease in the clinical outcome.
AMH, Oocyte quality, Oocyte quantity, Embryo quality and Clinical outcome
A COMPARATIVE STUDY BETWEEN EMBRYO QUALITY FERTILIZED WITH NORMAL EJACULATE SPERMS VS. TESA/PESA SPERMS
Our study aims to compare the quality of embryos fertilized using sperm obtained from testicular sperm aspiration (TESA) or percutaneous epididymal sperm aspiration (PESA) against those sourced from normal ejaculate in the intracytoplasmic sperm injection (ICSI). Male infertility is a significant issue. A particularly challenging form is azoospermia, a condition in which there are no sperm in the ejaculate. Azoospermia can be obstructive (OA) or non-obstructive (NOA). The causes for azoospermia vary; they can include genetic disorders, such as cystic fibrosis, or physical blockages. For patients with azoospermia, surgical methods like TESA & PESA offer hope for biological parenthood. This study's goal is to assess how effective these methods are in embryo fertilization by observing embryos till day 5 -blastocyst stage. These blastocysts serve as an important indicator of embryo viability.
TESA/PESA, male infertility, azoospermia